In conclusion, Serial Cloner is a very user-friendly and powerful Molecular biology tool. Fragments obtained by PCR, shRNA synthesis, can be easily assembled by selecting between the restriction sites.We can also ligate fragments using the ligate button.The overlapping regions selection box from SnapGene. You can indicate the specific length or specify the melting temperature. The SnapGene tools for Gibson Assembly and NEB HiFi Assembler allow you to customize the size of your homologous overlaps. Also, to create new PCR based fragments or synthetic adaptors. Table for determining overlapping regions for Gibson assembly. We can use either the graphics window or the text-based site usage windows, which makes it very easy to quickly extract a sub-sequence in a selection or in any restriction sites. Serial Cloner Tutorial - YouTube 0:00 / 2:56 Serial Cloner Tutorial Rissa B 23 subscribers Subscribe 18K views 7 years ago Very brief tutorial on how to use the Serial Cloner application.There is a web browser tool within the software to allow direct import of NCBI and EMBL.Sequence features are visible on the sequence map.They can be automatically found by using the scan feature. Features can be manually entered or imported.Note: If the DNA concentrations are low such. Ligase Buffer (1L/10L reaction for 10X buffer, and 2L/10L reaction for 5X buffer) 0.5-1L T4 DNA Ligase. Combine the following in a PCR or Eppendorf tube: Vector DNA. The fully graphical map makes it easy to extract and select a fragment or even show single, double, or multiple cutters in a single window. Use a ligation calculator to easily quantify how much vector and insert DNA to use. All the tools required to analyze the sequence and modify if any changes needed are available in a single window making it very easy to use.The software comes with a high-quality graphics display and has a very user-friendly interface to the front end, which makes the interface very intuitive. They are processed both in sequence and as a graphic map. Since version 2.0 of the software, it also handles Annotations and features. It can read and write files in several formats, making it portable across multiple users who use different Molecular Biology tools. 1,911 views Ligation-independent cloning is a form of molecular cloning that is able to be performed without the use of restriction endonucleas. It is available free for download and can be used across multiple Operating Systems, including Windows, Mac, and Linux. Serial Cloner is a Molecular Biology Tool which is a front end to help with DNA Cloning, Sequence Analysis, and Visualization.
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